FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558885
Sequences flagged as poor quality0
Sequence length35-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTA2500.00129428980.22115245
CGAGTAG16650.071.4578863
CCGAGTA17850.066.295642
AGTAGCT61900.065.9297565
GAGTAGC39050.063.2292024
GTAGCTG65300.062.6818586
TAGCTGG75200.056.7238967
AAGTGCT70850.056.5178875
GCTGGGA149750.054.915929
GCTACTC39850.054.257131
CCCGAGT22700.053.8239941
CCAAAGT81150.053.5220452
CTACTCG18450.052.698552
AAAGTGC75850.052.5392534
CAAAGTG81000.052.0417443
CCCAAAG90600.051.8246421
TACTCGG19000.051.509733
TTGGGAG94300.051.2047546
CAAGTAG28050.050.3977173
AGTGCTG79350.050.2129976
Produced by FastQC (version 0.11.5)