FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716974
Sequences flagged as poor quality0
Sequence length35-351
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT72500.070.7916265
CGAGTAG21400.069.6441043
GTAGCTG77000.066.021376
CCGAGTA23150.064.7100452
GAGTAGC49900.061.9633454
TAGCTGG85100.061.2125437
AAGTGCT79300.057.608035
GCTGGGA175450.057.396229
CTACTCG16500.056.163212
TACTCGG17550.055.556553
CAAGTAG34050.054.8153343
AGTGCTG90200.052.5766566
CCAAAGT94000.052.1830022
AAGTAGC35700.052.082934
CCCGAGT30000.051.6449931
CCAAGTA36550.051.4707642
AAAGTGC86900.051.44794
CAAAGTG92950.050.650263
CCCAAAG108750.050.3133621
GCTACTC39250.048.3643951
Produced by FastQC (version 0.11.5)