FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839495
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7050.034.0209122
TACTCGG7400.033.0240633
GCTGGGA74050.032.6861659
GAGTAGC22100.031.472294
CCCAAAG51500.031.2187461
GCTACTC18400.030.4928111
CGAGTAG9400.029.9973073
GTAGCTG36800.029.6277756
AGTAGCT35900.029.5850875
AAGTGCT44500.029.4647715
CGCCGTA404.785497E-429.37262258-59
AAAGTGC43750.029.325374
CCGAGTA9500.029.2074282
CCAAAGT48200.029.0759662
AGTGCTG47100.028.337166
GTGCTGG48300.028.2184227
CAAAGTG47450.028.0290993
TAGCTGG39100.028.006647
CTCGGGA8900.025.8740835
TGCTGGG56000.025.8490478
Produced by FastQC (version 0.11.5)