FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839495
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7800.041.605243
CCGAGTA8350.037.738262
TACTCGG9200.037.318883
CTACTCG9450.035.336222
GCTACTC22800.034.454961
CCCGAGT10350.033.178181
GCTGGGA73400.032.9149179
CGCGCTA600.004148156431.3546732
GGAGGCT55450.031.278839
CCCAAAG53050.030.8577271
AAAGTGC44000.030.5702674
TCGGGAG12550.030.3451586
AAGTGCT44750.030.0579175
CCAAAGT49600.029.9639422
AGTAGCT35150.029.8377045
GTAGCTG35550.029.3603676
GAGTAGC20750.029.2386654
TAGCTGG36950.029.1324737
TTGGGAG49900.028.266296
AGTGCTG47100.028.1498666
Produced by FastQC (version 0.11.5)