FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573955
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8550.037.376583
CCGAGTA9400.036.5199552
GAGTAGC21950.036.3974654
CCAAAGT46500.035.901342
TACTCGG8300.035.671323
AGTAGCT35750.034.7044075
AAAGTGC41850.034.6992264
AAGTGCT42800.034.6976475
GCTGGGA74500.034.0645529
CCCAAAG52800.034.012651
GCTACTC21150.032.983811
GTAGCTG37300.032.3803146
CTACTCG8450.032.2779922
CAAAGTG48350.031.9783613
TAGCTGG39500.031.886217
AGTGCTG45100.031.4693056
GGAGGCT61650.030.4162039
CCCGAGT11800.030.358551
GTGCTGG48100.029.897967
TTGGGAG49850.029.602016
Produced by FastQC (version 0.11.5)