FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573955
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10000.050.7956962
TACTCGG10050.050.542983
CGAGTAG9900.042.7573173
CCGAGTA10000.041.8594172
GCTACTC23600.041.0606581
GCTGGGA81300.039.370669
CCCGAGT11350.038.958541
CCCAAAG52650.038.865261
TAGCTGG40700.038.8101547
GAGTAGC24650.038.1599464
GTAGCTG39950.037.6596156
AGTAGCT39350.037.410575
TCGGGAG14550.037.160166
AAAGTGC46600.036.7375454
GGAGGCT64650.036.35119
ACTCGGG13850.036.3352974
CCAAAGT50650.036.3078422
CAAAGTG50200.035.790093
AAGTGCT47300.035.597265
TTGGGAG51600.035.443996
Produced by FastQC (version 0.11.5)