FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491791
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9650.037.038433
CCGAGTA10700.034.722392
CTACTCG10700.031.645722
CCCGAGT12450.030.9776251
TACTCGG10850.030.7747753
GAGTAGC24700.030.6545664
GCTGGGA87300.030.1498349
AGTAGCT40100.029.437145
CCCAAAG61750.029.3242841
AGTGCTG54900.027.4043466
AAAGTGC53100.027.2786254
GTAGCTG41250.027.2405416
CTCGGGA12850.027.0828825
AAGTGCT53900.027.0482525
GTGCTGG56550.026.350427
CCAAAGT62150.026.1062362
TAGCTGG45050.026.0859177
TTGGGAG52400.026.0200736
CAAAGTG60200.025.2331983
GGAGGCT69850.025.0988339
Produced by FastQC (version 0.11.5)