FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934216
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9400.043.495973
CTACTCG9400.042.5264972
CGAGTAG10350.039.5035863
AAAGTGC44500.036.8572584
CCGAGTA10850.036.8432352
GCTGGGA84900.036.7551359
CCCAAAG59300.035.766411
GCTACTC21150.034.987011
GTAGCTG42850.034.657236
AGTAGCT42550.034.5702445
AAGTGCT47450.034.3677335
TCGGGAG12000.034.0718466
TAGCTGG45250.034.0653537
CCAAAGT54650.033.9919052
CTCGGGA11850.032.9167825
GAGTAGC25950.032.7792364
CAAAGTG54350.032.6851043
AGTGCTG51200.032.676666
CCCGAGT13100.032.380891
GTGCTGG52050.031.5110117
Produced by FastQC (version 0.11.5)