FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934216
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11200.044.504463
CCGAGTA11750.043.2216722
TACTCGG9500.042.568713
CTACTCG9800.041.2655872
AAAGTGC52200.041.257534
AAGTGCT54200.040.515935
AGTAGCT43450.039.934195
GCTACTC24600.039.575371
GCTGGGA94400.039.532739
GAGTAGC26050.039.3511164
CCCAAAG63750.039.1745531
CCCGAGT13450.038.814181
CCAAAGT59350.038.664712
CAAAGTG59550.037.7452053
GTAGCTG45100.037.107636
TTGGGAG59850.037.0738456
GTGCTGG57350.036.964817
TCGGGAG12600.036.9363866
AGTGCTG57800.036.924776
TAGCTGG47150.036.5872467
Produced by FastQC (version 0.11.5)