FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3579686
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG555.2613017E-542.7603032
TACTCGG8100.042.390773
CTACTCG8500.041.5026472
CGAGTAG10400.038.4431573
CCGAGTA10600.037.2740752
GCTGGGA80200.036.5101139
GCTACTC25250.036.3316961
GAGTAGC26200.035.9040954
CCCGAGT13000.035.8264921
GGAGGCT63500.035.823769
AGTAGCT41750.035.5996635
GTAGCTG42600.033.6592986
TAGCTGG43250.033.1488087
CCCAAAG62650.032.5897521
TTGGGAG56200.031.2023536
AAAGTGC50950.030.556184
GTGCTGG53600.030.168097
AAGTGCT53500.029.9789095
CTCGGGA12100.029.5422425
AGTGCTG55200.029.127416
Produced by FastQC (version 0.11.5)