FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347523
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13400.045.9430923
CCGAGTA13750.045.4966132
TACTCGG9950.042.508243
CTACTCG10250.041.2998732
CCCGAGT16850.040.866391
AGTAGCT48700.040.529765
GAGTAGC31450.040.1962284
CCCAAAG66600.039.728481
GCTACTC24100.039.3361931
GTAGCTG50550.039.325386
GCTGGGA109850.038.6742639
AAAGTGC58350.038.498234
AAGTGCT60100.037.6118165
CCAAAGT66500.036.2848932
CAAAGTG65500.036.2329563
TAGCTGG55650.036.228127
AGTGCTG63250.035.6643566
GTGCTGG67550.033.60287
GGAGGCT75000.032.0820739
TCGGGAG14750.031.5425556
Produced by FastQC (version 0.11.5)