FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347523
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12350.051.7895743
CCGAGTA13100.048.8245242
CTACTCG12900.044.842082
TACTCGG13600.043.9172553
GAGTAGC31800.042.888544
AGTAGCT51600.042.1989485
CCCGAGT16350.041.1408351
GTAGCTG53100.040.1947566
CCCAAAG73450.039.5777851
GCTACTC29050.039.5092241
AAAGTGC60700.039.359124
AAGTGCT61050.038.979415
GCTGGGA112700.038.90899
CCAAAGT66500.038.4015352
TAGCTGG57100.038.1960567
TTGGGAG77400.037.658146
TCGGGAG17650.037.2899136
CAAAGTG66050.036.9542923
GGAGGCT88200.036.9280059
CTCGGGA17900.035.4691775
Produced by FastQC (version 0.11.5)