FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252004
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10500.033.149742
CGAGTAG9750.032.775893
GAGTAGC24300.031.9100784
GCTGGGA81000.031.3883979
CCCAAAG54900.031.0873871
AGTAGCT39850.029.9540545
TAGCTGG42950.029.8714547
GTAGCTG40150.029.379096
CTACTCG9100.028.9457152
CCCGAGT13400.028.8507461
TACTCGG9300.028.297973
AAAGTGC48650.027.626984
AAGTGCT50300.027.0944425
GCTACTC20750.027.0381431
CCAAAGT53750.026.9531732
AGTGCTG52350.026.75166
GTGCTGG53150.026.5261887
TCGATCG909.526682E-426.1086469
GGAGGCT56450.025.891279
CAAAGTG55000.025.462663
Produced by FastQC (version 0.11.5)