FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252004
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10700.039.1194653
CCGAGTA11650.037.5442732
GCTGGGA87000.036.6828659
CCCAAAG61600.036.1963271
CTACTCG9350.035.210572
AAAGTGC50050.035.144244
AGTAGCT40400.034.923675
TACTCGG10300.034.2460673
GAGTAGC25550.034.2380264
AGTGCTG54100.033.373396
AAGTGCT53750.033.07455
CCCGAGT14650.032.7513661
GCTACTC24200.032.6558381
CCAAAGT60500.032.2611162
GTAGCTG41900.032.0935946
CAAAGTG58950.032.072213
TAGCTGG45200.031.929067
GTGCTGG56550.031.1734497
CGCCGTA2300.030.6527594
TTGGGAG55850.030.1388176
Produced by FastQC (version 0.11.5)