FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199661
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11500.042.1165662
CGAGTAG10750.041.0949633
TACTCGG8300.038.5034833
CTACTCG8100.037.734792
CCCGAGT13300.037.2238041
GCTGGGA82050.037.116969
AGTAGCT40950.036.2662285
GAGTAGC28050.035.6874854
GTAGCTG42150.035.6797336
TAGCTGG45200.034.8323147
CGCGATA550.002718609334.179563
GCTACTC20350.032.2057571
CCCAAAG59700.032.065261
TCGCGTT753.2781056E-431.3312665
AAAGTGC48350.030.3268494
AAGTGCT50000.029.420065
CCAAAGT56900.029.25532
TTGGGAG54300.029.0809546
AGTGCTG52600.028.7699646
CAAAGTG54800.028.7298553
Produced by FastQC (version 0.11.5)