FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199661
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9000.047.029862
TACTCGG9450.043.7950023
CGAGTAG10000.042.7971763
ATTCGCG555.2654486E-542.754424
GCTACTC22650.039.457891
CCGAGTA11000.038.0514342
GCTGGGA85050.036.4741559
GGAGGCT63400.036.178149
AGTAGCT40650.035.8653345
CGGAGGT13050.035.6616487
TTGGGAG62950.035.6265756
GAGTAGC25450.035.480294
CCCGAGT13400.035.4539831
GTAGCTG41100.034.089916
CCCAAAG61000.033.929021
GGGAGGC82600.032.549578
TAGCTGG46300.032.3881727
CTCGGGA13800.032.375635
AAAGTGC50500.032.3155674
TCGGGAG13750.031.8003336
Produced by FastQC (version 0.11.5)