FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872099
Sequences flagged as poor quality0
Sequence length35-351
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT83800.073.9423145
CCGAGTA27000.071.441752
CGAGTAG26050.070.695743
GTAGCTG89150.069.35586
GAGTAGC58750.067.446524
ACTCGTA2450.002723623866.509125340-345
TAGCTGG98250.064.827867
AAGTGCT87850.062.5114335
GCTGGGA188400.060.7688459
AAAGTGC95400.057.5642474
CCAAAGT104500.057.0308072
CTACTCG19650.056.5290532
CAAGTAG34950.056.1172033
TACTCGG20050.055.7078133
CCCAAAG116450.055.0916671
CAAAGTG102500.055.068723
CCCGAGT35600.055.016941
AGTGCTG100200.054.873036
TTGGGAG104200.054.2340976
CCAAGTA37850.052.719732
Produced by FastQC (version 0.11.5)