FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872099
Sequences flagged as poor quality0
Sequence length35-251
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT78700.075.443035
GAGTAGC51750.071.482074
CGAGTAG22100.071.4548953
GTAGCTG84650.069.904026
CCGAGTA22700.069.019652
TAGCTGG90000.067.677687
CTACTCG25050.066.515852
TACTCGG25200.066.1187743
CGATAAG2050.003091107664.4206230-234
AAGTGCT88500.064.349145
GCTACTC52950.063.5327221
GCTGGGA188500.061.4541369
CCAAAGT99750.060.271452
TTACGCA2201.0072654E-760.028282230-234
AAAGTGC95350.058.878614
CAAGTAG34000.058.6970983
CAAAGTG100250.058.3573763
TTGGGAG118250.058.083576
TTTATCG3900.004877134757.406967245
AAGTAGC35200.057.402554
Produced by FastQC (version 0.11.5)