FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743634
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10700.050.0692443
CCGAGTA11650.050.0539862
GAGTAGC26150.044.5686954
AGTAGCT42100.042.3064965
CCCGAGT14200.041.4972421
TACTCGG9250.041.1522183
GTAGCTG43550.040.682086
CTACTCG9200.040.3814742
TAGCTGG45550.040.031447
GCTACTC22900.038.9066541
GCTGGGA86900.038.6132479
AAAGTGC44800.036.29514
CCCAAAG55100.036.01871
AAGTGCT47450.035.8527345
TCGGGAG12550.034.4503866
CCAAAGT53300.034.0567322
CAAAGTG51200.033.9610983
GGAGGCT62700.032.4547469
CTCGGGA12950.032.2975925
TTGGGAG52650.032.2224666
Produced by FastQC (version 0.11.5)