FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743634
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9050.049.3699762
CGAGTAG9350.048.7919353
TACTCGG9650.047.275093
CCGAGTA10150.046.799722
GCTACTC23850.045.557411
GAGTAGC23400.045.422664
AGTAGCT37800.044.044485
GTAGCTG39550.041.725926
TAGCTGG41900.041.3986637
GCTGGGA88700.040.325499
CCCAAAG57300.040.2232251
AAAGTGC48100.040.186184
CCCGAGT12150.039.8746071
AAGTGCT49350.038.977695
CCAAAGT54250.038.6654432
AGTGCTG49900.038.630356
CAAAGTG53600.037.642673
TCGGGAG13500.036.2197046
TTGGGAG59550.035.6863376
GGAGGCT70100.035.4025429
Produced by FastQC (version 0.11.5)