FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488719
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10800.042.6434443
CCGAGTA11300.041.2055632
AAAGTGC42250.040.4878464
GAGTAGC23250.040.223524
TACTCGG8500.039.8073733
GCTGGGA82650.039.4047939
AGTAGCT38600.039.081185
CCCAAAG49600.038.702241
CCCGAGT12450.038.641521
GTAGCTG39900.038.1612056
AAGTGCT46350.038.0216985
CTACTCG8950.037.8362922
CAAAGTG46350.037.8189163
TAGCTGG42150.037.574317
CCAAAGT47950.037.3710672
GCTACTC20150.036.0469931
AGTGCTG47850.036.044096
GTGCTGG49650.034.3594367
TTGGGAG47100.033.026086
CTCGGGA11850.031.3298785
Produced by FastQC (version 0.11.5)