FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684698
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT81450.054.8078655
GTAGCTG88950.051.7097436
CGAGTAG23150.051.2098273
CCGAGTA24600.050.4142382
AAGTGCT92850.049.464815
GAGTAGC55450.049.3546874
TAGCTGG95800.049.2157487
GCTGGGA188250.049.1919339
CCAAAGT106750.044.629772
AAAGTGC100850.044.3990974
AGTGCTG103100.044.3500066
CTACTCG21250.044.010612
TTGGGAG108250.043.1161426
CCCAAAG123500.042.9917831
TACTCGG21950.042.2816853
CAAAGTG108800.042.152763
GGAGGCT138700.039.903049
CCCGAGT32700.039.865681
GCTACTC47000.039.148681
ACGAACG2804.5141496E-738.858627340-345
Produced by FastQC (version 0.11.5)