FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684698
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21800.059.8074723
CCGAGTA23050.059.0361672
CTACTCG24800.055.6359372
AGTAGCT78900.055.510335
TACTCGG24950.055.047033
GAGTAGC50300.054.4834564
GCTACTC53750.052.0517961
GTAGCTG83300.051.7311946
AAGTGCT94300.050.3378035
CCCGAGT28950.049.8514941
GCTGGGA186300.049.447679
AAAGTGC95000.049.4339144
TAGCTGG92350.048.5756347
CCAAAGT109050.046.4897772
CAAAGTG104500.046.3329433
TTGGGAG120200.045.5367476
CCCAAAG124800.045.14061
AGTGCTG108350.044.384886
GGAGGCT148950.043.6241769
TCGGGAG33800.043.6203966
Produced by FastQC (version 0.11.5)