FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105498
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT40850.034.8581285
CCCAAAG60300.034.476761
CGAGTAG10900.034.060743
GCTGGGA87450.033.911279
CCGAGTA11600.032.8335882
GAGTAGC26150.032.7079434
GTAGCTG44300.031.8251916
CCAAAGT55550.031.7423112
AAAGTGC49750.031.7394714
CTACTCG8300.031.7249742
AAGTGCT50100.031.6115445
CCCGAGT13800.031.086191
TACTCGG8050.030.9409393
TAGCTGG47950.030.580217
GCTACTC20400.030.2723621
AGTGCTG53500.029.6904266
GGAGGCT62200.029.1656349
GTGCTGG53650.029.0832467
CAAAGTG59850.028.5033573
TTGGGAG50700.027.8078146
Produced by FastQC (version 0.11.5)