FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105498
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA352.929471E-453.7469983
CGAGTAG10750.044.622513
CTACTCG10450.044.1033942
CCGAGTA11150.043.8652652
TACTCGG11650.041.1752783
CCCGAGT13100.039.4992331
GCTGGGA87750.038.0320369
CCCAAAG65600.037.0009731
GAGTAGC23950.035.541474
AAAGTGC54900.035.464214
GCTACTC26650.035.3020631
AGTAGCT39900.035.2425
CCAAAGT61700.035.061862
AAGTGCT58550.034.5385255
CTCGGGA14800.034.3181845
AGTGCTG58700.034.2806436
GTAGCTG40800.034.2246936
GTGCTGG59300.034.008697
TAGCTGG44250.033.783237
CAAAGTG61600.033.2864953
Produced by FastQC (version 0.11.5)