FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4058230
Sequences flagged as poor quality0
Sequence length35-351
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC3402.4842194E-4121.49644340-345
AGTAGCT101200.067.217115
GTAGCTG107850.063.492616
CGAGTAG29700.062.1116143
GAGTAGC70200.060.9427454
CCGAGTA31750.060.5804752
TAGCTGG118650.059.733797
AAGTGCT111950.056.773425
GCTGGGA238000.055.8323259
TTAGCGC3000.006903426352.58886310-319
AAAGTGC120650.051.8748444
CTACTCG22650.050.8946042
CCAAAGT132700.050.3161852
CCCGAGT41450.049.7773481
CAAAGTG127500.049.7508243
AGTGCTG129250.049.4247256
TAACGCC4200.00889109949.325813340-345
TACTCGG24400.049.075683
CCCAAAG150150.047.4467161
CAAGTAG46100.046.6145553
Produced by FastQC (version 0.11.5)