FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4058230
Sequences flagged as poor quality0
Sequence length35-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27550.067.810623
AGTAGCT93200.065.72015
CCGAGTA29600.064.355872
GAGTAGC62250.063.3666344
GTAGCTG102750.059.895846
AAGTGCT114400.057.9315765
TAGCTGG112000.057.293787
CTACTCG27800.056.1842842
GCTGGGA233250.055.885899
GCTACTC61350.055.8174861
AAAGTGC118250.055.631054
CCAAAGT126700.055.015512
TACTCGG29100.053.2533573
CAAAGTG125950.053.2519383
AGTGCTG127150.052.7363626
TCGAATG6203.254542E-452.598347245
CCCAAAG146650.051.8397371
GTGAACG6403.8066754E-450.954647245
CCCGAGT37600.050.8323021
TTGGGAG142550.049.7454956
Produced by FastQC (version 0.11.5)