FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2869459
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11050.045.508373
CTACTCG8950.042.5602572
CCGAGTA12500.042.1357042
AGTAGCT46050.041.026665
GAGTAGC28950.040.584594
GTAGCTG46350.040.355536
TACTCGG9450.040.2831083
GCTGGGA94600.038.1553089
CCCGAGT14900.037.6506041
CGCGATA500.001702992737.597573
TAGCTGG50750.037.043237
GCTACTC21700.036.7145541
AAGTGCT48900.036.136725
CCCAAAG61100.035.9547271
AAAGTGC49750.035.519314
CCAAAGT54400.035.2698442
TCGCGAA550.002718544634.17960794
AGTGCTG52500.033.6587756
CAAAGTG54200.033.6435783
GGAGGCT67000.032.407999
Produced by FastQC (version 0.11.5)