FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2869459
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10950.042.9498253
AGTAGCT42950.042.484355
AAGTGCT52850.042.1787535
AAAGTGC52950.042.09914
CCGAGTA11650.041.9839172
CGAGTAG11150.041.7576333
CTACTCG10750.041.5614552
CCAAAGT59150.041.5041282
GCTACTC25500.040.579911
CCCAAAG66000.040.5505641
GCTGGGA99250.039.87569
CAAAGTG59400.039.745953
GTAGCTG45800.039.5229726
AGTGCTG57500.039.4942326
GAGTAGC26450.039.116274
TAGCTGG47350.039.116047
TCGGGAG15300.036.8760346
GTGCTGG62650.036.7666177
TTGGGAG64700.036.6252566
CCCGAGT14450.036.4568181
Produced by FastQC (version 0.11.5)