FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2723435
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9300.051.5807762
TACTCGG9650.047.2732053
CGCGAAC502.9993653E-547.0295332
CGAGTAG11200.046.6096273
CCGAGTA12000.045.0699652
GCTACTC23000.043.561441
CCCAAAG62250.043.2977941
GAGTAGC26100.041.984224
AGTAGCT42150.041.9527975
AAAGTGC50050.041.9084364
TCGCGAA450.001014274741.77715794
CCAAAGT58650.041.1358072
GCTGGGA95400.040.9419029
CCCGAGT14200.040.4130821
AAGTGCT52050.040.117415
CAAAGTG57500.039.5048073
GTAGCTG44600.039.006216
TAGCTGG47650.038.7697337
AGTGCTG55850.037.1263586
ACTCGGG12900.036.4574
Produced by FastQC (version 0.11.5)