FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417071
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11400.030.0934723
CCGAGTA11700.029.3414422
TACTCGG11000.027.7699413
CTACTCG11150.026.5711362
GAGTAGC29550.026.4000574
GCTGGGA99600.025.9992799
CCCAAAG70050.025.4362181
CCCGAGT15950.024.82491
AGTAGCT49300.023.9266055
GGAGGCT73700.023.2752519
AAAGTGC61700.023.2310564
TTGGGAG70000.023.0948246
GTAGCTG50350.023.054296
GGGAGGC85250.022.8231188
CCAAAGT72400.022.6689452
AAGTGCT64000.022.6164825
AGTGCTG64950.022.430396
TAGCTGG54550.022.3138287
GCTACTC26900.022.0793931
CGTCGAG1754.3316722E-621.5486891
Produced by FastQC (version 0.11.5)