FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417071
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11700.036.1773762
CGAGTAG12650.033.832283
TACTCGG12850.032.9397133
CCGAGTA13250.031.9453052
GCTGGGA107800.029.8230489
GCTACTC31000.029.7422081
GAGTAGC31200.028.4896814
CCCAAAG80500.027.6988241
CCCGAGT18450.027.2813741
AGTAGCT52750.026.6577955
AAAGTGC67950.026.6472034
AAGTGCT70500.026.3501745
GTGCTGG70450.025.8219457
GGAGGCT78800.025.6482059
CCAAAGT78900.025.3333342
GTAGCTG52900.025.330096
CGGAGGT15800.025.2883687
AGTGCTG73100.024.9552486
CAAAGTG78050.024.6451113
CTCGGGA16800.024.634795
Produced by FastQC (version 0.11.5)