FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3557186
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11400.031.7427853
CCGAGTA12100.030.701342
TACTCGG9850.029.581123
GCTGGGA87950.029.5506329
GAGTAGC26950.028.7729474
CCCGAGT14700.028.545131
GTAGCTG44550.027.5328986
AGTAGCT44100.027.3875835
CTACTCG9500.027.2241732
CCCAAAG59200.026.520511
GCTACTC23150.026.475961
TAGCTGG49500.026.2048327
AAAGTGC50750.024.724894
CCAAAGT57000.024.6667522
GGAGGCT60650.024.5643869
GTGCTGG54100.024.1504387
TTGGGAG55550.024.1112636
AAGTGCT53200.023.5862435
CGACCGT802.0407424E-623.50071574-75
CGGGTTC7850.023.3492229
Produced by FastQC (version 0.11.5)