FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3557186
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10300.038.3544923
CCGAGTA11450.034.5022962
GCTGGGA87900.031.656489
TACTCGG11000.031.2107623
CCCGAGT14300.030.2595631
GAGTAGC27650.030.1055264
CCCAAAG65000.030.1016861
AGTAGCT42550.029.1791385
CTACTCG10350.029.0813032
TTGGGAG63600.028.3147036
CCAAAGT61800.028.3092692
GGAGGCT70200.028.1886849
GTAGCTG43500.028.103116
AAAGTGC55250.027.9196284
AAGTGCT55050.027.6793445
CAAAGTG60700.027.6600993
GCTACTC25850.027.474341
GTGCTGG57850.026.7344477
TAGCTGG48200.026.6252887
GGGAGGC84100.026.0450888
Produced by FastQC (version 0.11.5)