FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379544
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10200.043.3360062
CGAGTAG9200.042.9351163
CCCGAGT12450.038.533251
TACTCGG8600.037.1819043
CTACTCG8650.035.879722
TCGCGTT801.1552362E-535.268135
GCTGGGA82850.034.3776559
GCTACTC22850.033.5510561
AGTAGCT37900.033.500075
CCCAAAG60000.033.080041
GAGTAGC24900.032.8602684
GGAGGCT65050.032.296579
TTGGGAG60100.031.6814676
AAAGTGC52250.031.319454
GTGCTGG55350.031.0830677
TAGCTGG42400.030.7096147
AAGTGCT54550.030.6885555
AGTGCTG55600.030.2714316
CCAAAGT60050.030.148722
GTAGCTG41400.029.866216
Produced by FastQC (version 0.11.5)