FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3471529
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT1250.006757763147.32716245
TTAGCGC1750.001578572976.31792200-204
CTAGACG4300.003123585164.241486245
TTGACGC2900.00403511560.227837235-239
CGAGTAG19400.058.4030573
CCGAGTA21050.057.5087432
AGTAGCT63450.055.0735975
CTACTCG16650.051.9330372
GAGTAGC44000.051.9067154
GCTGGGA145700.051.1851239
AAGTGCT83000.050.4355165
CCCAAAG101400.049.231871
AAAGTGC84450.049.216484
GTAGCTG69100.049.182626
TACTCGG17800.048.9121323
CCAAAGT93600.048.2291952
TAGCTGG72350.047.299547
CAAAGTG92500.046.8679963
CCCGAGT26400.046.7654651
TTGGGAG96750.046.0962376
Produced by FastQC (version 0.11.5)