FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4010742
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA98550.035.479829
CGAGTAG12000.034.0715833
AAAGTGC57400.033.240574
CCCAAAG71650.032.9744951
CCGAGTA13200.032.7812652
AAGTGCT60700.031.7431095
AGTGCTG63750.031.035326
GAGTAGC31600.030.6361684
GTGCTGG63100.030.4620487
AGTAGCT50900.030.3761315
TAGCTGG56000.030.295937
CTACTCG9850.030.082652
TACTCGG9750.029.8841843
GTAGCTG52400.029.6859556
CCCGAGT15750.029.6422581
CAAAGTG67650.029.5934853
CCAAAGT69500.028.9648152
TGCTGGG72900.027.914228
GCTACTC26550.027.3534951
GGAGGCT69950.026.00099
Produced by FastQC (version 0.11.5)