FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4010742
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12250.040.6903843
CCGAGTA12800.040.411482
TACTCGG11100.038.5514223
CTACTCG11300.037.4529532
GAGTAGC30050.036.1479844
CCCAAAG75500.035.880391
CCCGAGT16400.035.8465841
GCTGGGA103200.035.660949
GGAGGCT79450.033.956959
CCAAAGT72050.033.8731352
GCTACTC28200.033.688671
AAAGTGC63100.033.535114
CGGAGGT19550.032.9395037
AGTAGCT50350.032.5943685
TTGGGAG72700.032.460556
GTAGCTG52450.031.1903516
AAGTGCT68150.030.9811155
GTGCTGG67900.030.7366437
TAGCTGG54550.030.3313067
CAAAGTG75250.030.3079993
Produced by FastQC (version 0.11.5)