FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3833182
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10250.027.9675983
CGCGAGT700.00869863126.9451681
CCGAGTA12100.026.43442
GAGTAGC27000.025.063864
GCTGGGA84350.024.9054479
CCCGAGT14650.024.4621661
AGTAGCT42300.023.8862135
TAGCTGG45600.023.7047217
GCTACTC22700.023.6808831
CCCAAAG60250.022.853081
GTAGCTG44400.022.5447736
ATGCGCG1050.002338003822.3784474
GGAGGCT59450.021.8187129
GTGCTGG50750.021.7622457
AAAGTGC51750.021.2497944
AGTGCTG53250.021.004226
TTGGGAG58950.020.5676696
CCAAAGT59850.020.1983032
AAGTGCT53450.019.8705545
TGCTGGG60850.019.6175618
Produced by FastQC (version 0.11.5)