FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3833182
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10500.034.039273
CTACTCG10300.032.8738982
CGAGTAG10350.030.8975853
CCGAGTA11700.029.744172
GCTGGGA84050.029.1907339
CCCGAGT14050.028.4562681
CCCAAAG61750.028.0314941
GCTACTC25350.027.090081
CGTTGCG909.488774E-426.1266332
CGGTACG909.488774E-426.1266332
GGAGGCT66700.026.0728249
GAGTAGC25700.025.6183344
TTGGGAG63350.025.5288776
AGTAGCT44600.025.2007435
TAGCTGG44450.024.9569857
CGGAGGT14700.024.9418777
GGGAGGC76350.024.6858188
GTAGCTG43350.024.618226
AAGTGCT58150.024.5043535
AAAGTGC57100.024.4611194
Produced by FastQC (version 0.11.5)