FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030615
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10650.045.483563
CCGAGTA10600.044.810762
CTACTCG10650.044.6003842
CGAGTAG10550.042.7942733
CCCAAAG63250.039.563041
GCTACTC25650.038.5097271
AAAGTGC52600.038.3564454
CTCGGGA12900.038.279515
CCAAAGT62100.036.275282
CCCGAGT14050.035.8216861
GCTGGGA90450.035.751039
CAAAGTG60500.035.2135353
AAGTGCT57350.034.933575
GAGTAGC26450.034.8495564
AGTAGCT41650.034.6649175
AGTGCTG57750.034.2753756
TCGGGAG15100.034.2505726
GTAGCTG42350.034.082946
GTGCTGG58400.033.406427
ACTCGGG14700.032.9523744
Produced by FastQC (version 0.11.5)