FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712200
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14750.040.7826463
CCGAGTA15100.039.2519042
GCTGGGA117300.038.2225389
CCCAAAG76450.037.8160131
AGTAGCT60700.037.4726265
GTAGCTG61050.037.2577936
GAGTAGC35850.037.0983624
CCAAAGT69850.036.7699782
TAGCTGG63950.036.7450337
CTACTCG10450.036.461612
AAAGTGC63100.035.8984034
AAGTGCT65250.035.507815
TACTCGG11050.034.4492843
CAAAGTG69950.033.9954073
AGTGCTG67300.033.797756
CCCGAGT18600.033.7234081
GCTACTC28150.032.837531
GTGCTGG70300.031.754667
CGGGTTC10300.031.0270779
GGAGGCT83200.030.1073889
Produced by FastQC (version 0.11.5)