FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712200
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG13350.050.0244983
CTACTCG14150.046.5315252
CGAGTAG13900.046.3533863
CCGAGTA14750.044.0010222
AGTAGCT53150.041.0573045
GAGTAGC33750.040.9684224
AAAGTGC65650.040.5468754
CCCAAAG79550.039.975461
AAGTGCT68350.039.5644385
GCTGGGA120900.039.3807229
CCAAAGT73500.039.0317652
CCCGAGT17200.038.8371051
CAAAGTG73750.038.1979873
GTAGCTG56550.038.077466
AGTGCTG71950.037.1806226
ACTCGGG18600.036.157534
TAGCTGG63300.035.7919047
CTCGGGA19350.035.7282755
TTGGGAG81350.035.4850966
GTGCTGG75500.034.9889457
Produced by FastQC (version 0.11.5)