FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654076
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12900.043.35353
CCGAGTA13850.040.407442
CTACTCG12750.036.147652
GCTGGGA105300.035.751559
TACTCGG12850.035.110343
CCCGAGT16100.034.8453671
CCCAAAG75250.034.7704621
GAGTAGC30750.034.540634
AAAGTGC60450.034.0521664
AGTAGCT53700.033.6940655
GCTACTC29850.033.640251
TCGCGTT702.1868446E-433.569045
AAGTGCT62800.033.2269365
CCAAAGT69250.033.1408962
CAAAGTG67200.032.5200083
AGTGCTG65150.032.2448276
GTAGCTG55300.032.039316
GTGCTGG68050.031.4249047
TAGCTGG58200.031.0905537
TGCTGGG76500.030.0426458
Produced by FastQC (version 0.11.5)