FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181606
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12700.046.6258933
CCGAGTA14050.043.179242
CTACTCG10200.036.4240762
GAGTAGC33000.035.887814
CCCGAGT17800.035.7481421
TACTCGG10750.035.4108963
GCTGGGA99950.035.3598989
AGTAGCT51550.033.184335
GTAGCTG51250.032.6449856
CCCAAAG72100.031.5755961
GCTACTC27150.030.7286241
TAGCTGG54900.030.3900877
AAAGTGC61650.030.0347164
AGTGCTG65050.029.9820376
AAGTGCT65050.029.7652995
GTGCTGG67350.029.5872217
CTCGGGA14150.029.2271585
CGGAGGT13950.028.9734487
GGAGGCT76250.028.41439
CCAAAGT70050.028.3312642
Produced by FastQC (version 0.11.5)