FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181606
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12450.043.811643
CCGAGTA13600.040.106982
CTACTCG11450.035.7284932
TACTCGG12000.035.2664833
GCTGGGA102150.034.462519
CCCAAAG75350.033.017251
CCCGAGT17250.032.716031
CGCCGTA1457.4578566E-1032.4142194
AAAGTGC64700.031.759824
GAGTAGC31250.031.4482984
AGTAGCT51300.031.4396465
GCTACTC30900.031.3528611
AAGTGCT66850.030.9493965
GGAGGCT84350.030.8136759
CCAAAGT74300.030.7573092
AGTGCTG67900.030.2557966
GTGCTGG67850.030.1362787
TTGGGAG73900.029.5804966
GTAGCTG53750.029.2997076
TAGCTGG56250.029.247977
Produced by FastQC (version 0.11.5)