FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838806
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTC1250.007359901143.18597280-289
AGTAGCT75150.051.4322245
CGAGTAG21400.049.152523
GTAGCTG79100.048.623556
CCGAGTA22750.048.4861072
GAGTAGC48950.048.2825934
GCTGGGA168650.047.5299039
TAGCTGG88200.046.052397
AAGTGCT88050.045.0484055
CTACTCG18800.044.5108152
AAAGTGC93450.042.445294
CCCGAGT28150.041.2882581
TACTCGG19450.041.048983
CCCAAAG118550.041.0375141
GCTACTC42900.040.8606871
TAACGAG3808.553543E-740.835587340-345
AGTGCTG99050.040.3014376
CGACCGT2703.0246546E-440.226765300-309
CCAAAGT105500.040.139642
CAAAGTG103350.039.6684533
Produced by FastQC (version 0.11.5)