FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4545696
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT101100.056.7797135
GTAGCTG109300.051.8656656
GAGTAGC70650.050.793784
CGAGTAG28950.049.853723
CCGAGTA30900.049.469412
AAGTGCT112550.049.3860475
TAGCTGG120550.049.18427
GCTGGGA234650.048.7906729
CCAAAGT132900.045.665062
AAAGTGC122050.045.275664
CAAAGTG131350.043.7042663
CCCAAAG158100.043.065961
CTACTCG26100.042.617012
TTGGGAG139700.042.3475846
AGTGCTG130850.042.330126
TACTCGG27050.041.098133
CCCGAGT40200.040.033231
GGAGGCT163750.038.8289389
TGAGTAG52700.038.1189653
GTGCTGG147950.037.438867
Produced by FastQC (version 0.11.5)