FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3722699
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15300.044.8456953
CCGAGTA16100.043.528112
CTACTCG11650.042.390922
GAGTAGC36900.042.0287934
CCCAAAG81800.041.967751
TACTCGG12350.041.8586353
CGGTACG450.001010674641.8077622
AAAGTGC68700.040.9759864
AGTAGCT59350.040.7006845
CCAAAGT77300.040.0364762
CAAAGTG74100.039.8925443
AAGTGCT70050.039.6495865
GCTGGGA130450.039.197279
GTAGCTG62250.039.1820646
CCCGAGT18800.039.129511
TAGCTGG66100.038.3939977
AGTGCTG75400.036.7115976
GCTACTC29600.036.1636121
CTCGGGA17000.034.2793125
TCGGGAG18050.033.8474126
Produced by FastQC (version 0.11.5)