FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3722699
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17050.047.4428183
CCGAGTA17750.046.366692
AGTAGCT60200.046.325365
TACTCGG15850.045.397153
GTAGCTG62750.044.729976
GAGTAGC38150.044.3780864
CTACTCG15900.044.071272
GCTACTC37450.043.332091
AAAGTGC70200.042.472984
CCCGAGT19750.042.1549451
GCTGGGA132800.041.801949
TAGCTGG68700.041.740037
AAGTGCT71350.041.458855
CCCAAAG85400.041.1989441
CAAAGTG77850.040.3537833
CCAAAGT80800.040.044562
AGTGCTG76300.038.511776
TTGGGAG82850.036.885776
TCGGGAG22500.036.5673566
GGAGGCT105650.035.948979
Produced by FastQC (version 0.11.5)